Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly

Expression of antibody fragments by periplasmic secretion in Escherichia coli.

Antibody-based drugs are increasingly used in the clinic, and their importance is set to escalate in the coming years as more drugs in this class progress through clinical trials. Although many such drugs utilize whole antibodies, others exploit fragments, e.g., fragment antigen binding (Fab') or single-chain fragment variable (scFv), which retain the antigen-binding specificity without the fra...

Novel multispecific heterodimeric antibody format allowing modular assembly of variable domain fragments

Multispecific antibody formats provide a promising platform for the development of novel therapeutic concepts that could facilitate the generation of safer, more effective pharmaceuticals. However, the production and use of such antibody-based multispecifics is often made complicated by: 1) the instability of the antibody fragments of which they consist, 2) undesired inter-subunit associations,...

Periplasmic expression of D1.3 Fab antibody in E. coli

Efficient Expression of Antibody Fragments with the Brevibacillus Expression System

Antibodies, owing to their capability to bind specifically to a target molecule, have been and will continue to be applied in various areas, including research, diagnosis and therapy. In particular, antibody fragments, which are size-reduced antibodies comprising functional variable domains, are suited for production in bacteria. They also are useful in applications requiring intracellular deli...

Enantioselective antibody fragments

Antibodies have a unique ability to bind to a wide variety of different molecules, ranging from large protein antigens to low molecular weight organic molecules. The specificity and affinity of the binding varies between antibodies and can be utilised in many applications. Recombinant antibody fragments have advantages over traditional polyand monoclonal antibodies in production and immobilisat...